• Patent number: 11110274
    Abstract: Described is a low voltage, pulsed electrical stimulation device for reducing inflammation in a subject, which can be useful in the treatment of concussions, traumatic brain injury, cancer, and so forth.
    Type: Grant
    Filed: September 20, 2018
    Date of Patent: September 7, 2021
    Assignee: LEONHARDT VENTURES LLC
    Inventor: Howard J. Leonhardt
  • Publication number: 20210236817
    Abstract: Described is a low voltage, pulsed electrical stimulation device for controlling expression of, for example, follistatin, a muscle formation promotion protein, by tissues. Epicardial stimulation is especially useful for heart treatment. Follistatin controlled release is also useful for treating other ailments, such as erectile dysfunction, aortic aneurysm, and failing heart valves.
    Type: Application
    Filed: March 29, 2021
    Publication date: August 5, 2021
    Inventors: Howard J. Leonhardt, Jorge Genovese
  • Publication number: 20210228870
    Abstract: Described is a low voltage, pulsed electrical stimulation device for controlling expression of Collagen Type XVII Alpha 1 chain (COL 17A1), a useful protein, by tissues. Also described are methods of COL17A1 in cells.
    Type: Application
    Filed: January 28, 2021
    Publication date: July 29, 2021
    Inventor: Howard J. Leonhardt
  • Patent number: 10960206
    Abstract: Described is a low voltage, pulsed electrical stimulation device for controlling expression of, for example, follistatin, a muscle formation promotion protein, by tissues. Epicardial stimulation is especially useful for heart treatment. Follistatin controlled release is also useful for treating other ailments, such as erectile dysfunction, aortic aneurysm, and failing heart valves.
    Type: Grant
    Filed: November 14, 2017
    Date of Patent: March 30, 2021
    Assignee: LEONHARDT VENTURES LLC
    Inventors: Howard J. Leonhardt, Jorge Genovese
  • Publication number: 20200324106
    Abstract: Described is a low voltage, pulsed electrical stimulation device for controlling expression of sonic hedgehog (“Shh”), a useful protein, by tissues. Also described are methods of enhancing expression of sonic hedgehog in cells, particularly a method of stimulating the expression and/or release of Shh in a cell having a gene encoding Shh, wherein the method includes applying a bioelectric signal of less than 50 Hz (e.g., 5 Hz, 10 Hz, or 20 Hz) at a pulse width duration of, e.g., 1 ms, to the cell (e.g., directly, indirectly, or wirelessly), and wherein the amount of Shh expression enhanced by this bioelectric signal is greater than that seen with a prior art bioelectric muscle stimulation or bioelectric muscle contraction alone as may be determined by, e.g., by an analysis of the upregulation of mRNA level/GAPDH fold gene expression in the cell.
    Type: Application
    Filed: April 13, 2020
    Publication date: October 15, 2020
    Inventor: Howard J. Leonhardt
  • Publication number: 20200289826
    Abstract: Described is a low voltage, pulsed electrical stimulation device for controlling expression of klotho, a useful protein, by tissues. Also described are methods of enhancing expression of klotho in cells.
    Type: Application
    Filed: March 13, 2019
    Publication date: September 17, 2020
    Inventor: Howard J. Leonhardt
  • Patent number: 10646644
    Abstract: Described is a low voltage, pulsed electrical stimulation device for controlling expression of, for example, follistatin, a muscle formation promotion protein, by tissues. Epicardial stimulation is especially useful for heart treatment. Follistatin controlled release is also useful for treating other ailments, such as erectile dysfunction, aortic aneurysm, and failing heart valves.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: May 12, 2020
    Assignee: CalXStars Business Accelerator, Inc.
    Inventors: Howard J. Leonhardt, Jorge Genovese
  • Publication number: 20200000709
    Abstract: Means and methods utilizing a combination of bioelectrical stimulator and platelet-rich fibrin for accelerated tissue or wound healing and regeneration is described. The system bioelectrically stimulates the centrifuge, test tube, and/or subject to produce enhanced levels of, e.g., SDF, PDGF, HGF, VEGF, IGF, Sonic hedgehog, klotho, and/or tropoelastin. The described system produces much higher levels of regenerative proteins delivered over an extended period of time.
    Type: Application
    Filed: July 1, 2019
    Publication date: January 2, 2020
    Inventors: Howard J. Leonhardt, Richard J. Miron, Valerie Kanter
1. Bioelectric InStim inflammation treatment 2X a week for 40 minutes each for 3 weeks prior to stem cell injections. Reads inflammation and real time adjusts bioelectric signals to control anti-inflammatory cytokines release on demand. 2. Bioelectric stem cell recruiting and protein expression treatment in clinic for regeneration 2X a week for 20 to 40 minutes for 16 weeks. Includes SDF-1, IGF-1, HGF, EGF, PDGF, VEGF, HIF1a, eNOS, Activin, Follistatin, Tropoelastin, BMPs. 3. Adipose or bone marrow derived stem cell injections with may be suspended (where regulatory agencies accept) in a growth factor cocktail, amniotic fluid, PRF, nutrient hydrogel, selected exosomes, micro RNAs, selected alkaloids and joint matrix- 1X a month for 6 months or until joint is recovered. 4. PRP, PRF, Amniotic Fluid injections – 1X per month for 6 months or until joint is recovered. 5. @ home stimulation with portable microcurrent leg, knee, calf and foot electric stimulation microcurrent garments (joint wrap) – use everyday until recovery. 6. Electro-acupuncture with Dr. Y. King Liu patented knee brace wrap one treatment day a month for 6 to 18 months or until joint is recovered.
Treatment protocol will vary depending on regulatory constraints in certain countries and physician preference.
OrthoStim Bioelectric Knee Stimulation

Knee brace having electromagnetic stimulators for continual electro-acupunctural stimulation; in vivo and in situ tissue engineering

Publication number: 20170259058 Abstract: An acupuncture stimulation system comprises a skin overlaying component and a strap having hook-and-loop fastener material, the strap can be wrapped around the skin overlaying component to snugly secure a portion of the system. Type: Application Filed: May 22, 2017 Publication date: September 14, 2017 Inventor: Y. King Liu

Knee brace having three stimulators for continual electro-acupunctural stimulation; in vivo and in situ tissue engineering

Patent number: 9687376 Abstract: A knee brace for continual electro-acupuncture stimulation system comprises a first circuit having a first circuit having a first electrode configured to electrically coupled to acupuncture point “Heting (S 156)” and a second electrode configured to electrically coupled to acupuncture point “Bladder 40”, a second circuit having a third electrode configured to electrically coupled to acupuncture point “Spleen 10” and a fourth electrode configured to electrically coupled to acupuncture point “Hsiyen (S 145)”, and a third circuit having a fifth electrode configured to electrically coupled to acupuncture point “Stomach 34” and a sixth electrode configured to electrically coupled to acupuncture point “Stomach 35”. The first, third, and fifth electrodes are connected to a polarity of voltage, and the second, fourth, and sixth electrodes are connected to an opposite polarity of voltage, such that electric currents flow in a body of a patient to achieve analgesia, cartilage repair and regeneration in the knee joint. Type: Grant Filed: August 6, 2013 Date of Patent: June 27, 2017 Inventor: Y. King Liu

Knee brace for continual electro-acupunctural stimulation; in vivo and in situ tissue engineering

Patent number: 9629742 Abstract: A knee brace for continual electro-acupuncture stimulation system comprises: a first electrode configured to electrically coupled to acupuncture point Heting “(S 156)”; a second electrode configured to electrically coupled to acupuncture point “Spleen 10 ”; a third electrode configured to electrically coupled to acupuncture point “Stomach 34 ”; a fourth electrode configured to electrically coupled to acupuncture point “Hsiyen (S 145)”; a fifth electrode configured to electrically coupled to acupuncture point “Stomach 35 ”; a sixth electrode configured to electrically coupled to acupuncture point “Bladder 40 ”. The first, second, and third electrodes are connected to a polarity of voltage, and the fourth, fifth, and sixth electrodes are connected to an opposite polarity of voltage, such that electric currents flow in a body of a patient to achieve analgesia, cartilage repair and regeneration in the knee joint. Type: Grant Filed: December 24, 2012 Date of Patent: April 25, 2017 Inventor: Y. King Liu

Percutaneous continual electro-acupuncture stimulation for in vivo and in situ tissue engineering

Patent number: 9440069 Abstract: The invention includes an electro-acupuncture stimulation system for in vivo and in situ analgesia and tissue repair and regeneration. Electrodes, which can be acupuncture needles, are percutaneously implanted that deliver a pulsed electrical current that creates an electrical field, which envelopes the targeted tissue and restores cell-generating homeostasis to the affected tissue and thereby promotes analgesia and tissue re-growth in otherwise debilitated or deteriorating tissue. Methods and apparatuses are also disclosed that may include a needle locking system and acupuncture-needle assemblies for long-term in situ electrical stimulation. Type: Grant Filed: November 25, 2009 Date of Patent: September 13, 2016 Inventor: Y. King Liu